目的 :探讨中药消癃通闭对大鼠前列腺平滑肌中一氧化氮合酶 (NOS)及上皮细胞凋亡的影响。方法 :用还原型辅酶Ⅱ组化染色结合形态学定量分析方法 ,检测各组大鼠前列腺中NOS神经含量 ;采用免疫组化原位末端标记染色法 ,检测大鼠前列腺上皮细胞的凋亡百分率。结果 :消癃通闭高剂量组在用药 3周后 ,前列腺组织中NOS神经的长度密度 (Lv)为 0 113± 0 0 2 3,与其他 3组 (消癃通闭低剂量组、保列治组及对照组 )比较差异均有显著性 (P <0 0 1)。消癃通闭高剂量组及保列治组在实验第 7天时 ,凋亡细胞百分率达到高峰 (分别为 9 2 7%、5 6 5 % ) ,第 14天时稍减少 ,第 2 1天时有所恢复。去势组大鼠在第 7天时凋亡细胞即出现 ,到第 14天时凋亡细胞百分率达到高峰 ,与其他组比较差异有显著性。结论 :中药消癃通闭可增加模拟前列腺增生大鼠前列腺中NOS含量 ,在较短时间内促进大鼠前列腺上皮细胞凋亡。 Objective : To investigate the effect of traditional Chinese medicine for removing phlegm and blood stasis on the expression of nitric oxide synthase (NOS) and epithelial cell apoptosis in rat prostate smooth muscle. METHODS: The NOS neurons in the prostate of rats in each group were detected by reducing coenzyme II histochemical staining combined with quantitative morphometric analysis. The percentage of apoptosis in rat prostate epithelial cells was detected by immunohistochemistry in situ end labeling. Results: The length density (Lv) of NOS nerves in the prostate tissue was 0 113 ± 0 0 2 3 in the high-dose group after 3 weeks of treatment, and it was compared with the other three groups (low-dose group and preserved group). The difference between the control group and the control group was significant (P <0 01). The percentage of apoptotic cells peaked at the 7th day of the experiment on the high-dose group and the Paul group (92.7%, 56.5 %, respectively), and decreased slightly on the 14th day, and on the 21st day. restore. In the ovariectomized rats, apoptotic cells appeared on the 7th day, and the percentage of apoptotic cells peaked on the 14th day. There was a significant difference compared with other groups. Conclusion : Traditional Chinese medicine can increase the expression of NOS in the prostate of rats with benign prostatic hyperplasia, and promote the apoptosis of rat prostate epithelial cells in a short time.