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对混合样品STR图谱的结果进行解释。实验模拟二组分DNA混合样品 ,复合扩增荧光检测 10个基因座 ,比较混合样品谱带 ,计算等位基因峰面积比。结果发现 :二组分DNA混合样品的等位基因数增加 ,样品的混合比例不同就出现峰面积的不平衡。在等位基因峰面积比值与样品组分混合比例接近时 ,可由峰面积比值推断混合样品的混合比例。在混合比例为 1∶2 0时 ,基本上检测不到来自少量混合成分的等位基因 ,表现为单一组分图谱 ;在混合比例为 1∶10时 ,含量低的组分的等位基因峰面积接近与主要组分的“Stutter”峰面积 ,与来自主要组分的等位基因峰面积差异很明显。能检出混合样品中少量成分等位基因的最高混合比例为 1∶10
The results of STR maps of mixed samples are explained. Experimental simulation of two-component DNA mixed samples, multiplex amplification fluorescence detection of 10 loci, mixed sample bands were compared to calculate the allele peak area ratio. The results showed that: the two-component DNA mixed samples increased the number of alleles, the sample mixture of different peak area appears uneven. When the allele peak area ratio is close to the sample component mixing ratio, the mixing ratio of the mixed sample can be inferred from the peak area ratio. At a mixing ratio of 1: 20, essentially no detectable allele from a small amount of mixed components appeared as a single component map; at a mixing ratio of 1: 10, the allelic peak of a low content component The area of the “Stutter” peak close to the major component is quite different from the peak area of the allele from the major component. Able to detect mixed samples in a small amount of composition of the highest allele ratio of 1:10