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本文报告,与主要组织相容性复合物Ⅱ类分子有关的对抗原的无应答可通过将抗原与能被无应答者Ⅱ类分子识别的T辅助细胞决定簇(TDh)联结而得以克服.作者对4周龄SJL/J(H-2~S)或B10M(H2~f)雌性小鼠(已知对HBsAg的S区无应答)腹腔内注射200μl乳剂,含100μl弗氏完全佐剂、50μl重组乙型肝炎疫苗(含1μg HBsAg)和50μlPBS,或注射1μgHBsAg和60μgFIS混合物,FIS为在Balb/c 小鼠中具有TDh特性的抹香鲸肌红蛋白中106～118位氨基酸肽,可被SJL/J小鼠的Ⅱ类分子识别.30和45天时分别用等量HBsAg加弗氏不完全佐剂作加强接种.分别于首针后30、45和60天从小鼠后眼窝采血.用ELISA测定抗体滴度.
This paper reports that non-responsiveness to antigens associated with major histocompatibility complex class II molecules can be overcome by linking the antigen to the T helper cell subset (TDh) that can be recognized by non-responder class II molecules. Four-week-old female SJL / J (H-2-S) or B10M (H2-f) mice, known to have no response to the S-region of HBsAg, were injected intraperitoneally with 200 μl of an emulsion containing 100 μl Freund’s complete adjuvant, Recombinant hepatitis B vaccine (containing 1 μg of HBsAg) and 50 μl of PBS or 1 μg of HBsAg and 60 μg of FIS mixture were injected at the amino acid positions 106-118 of the sperm whale myoglobin with TDh profile in Balb / c mice, Mouse class II molecular recognition .30 and 45 days were treated with an equal amount of incomplete HBsAg plus Freund’s incomplete adjuvant vaccination were collected 30, 45 and 60 days after the first needle back from the litter of mice. degree.