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表面等离子体共振(Surface Plasmon Resonance,SPR)技术是利用金属薄膜光学耦合产生的物理光学现象建立的一种非常灵敏的光学分析手段.近年发展的电化学表面等离子体共振(Electrochemical Surface Plasmon Resonance,EC-SPR)是将时间分辨表面等离子体共振光谱技术与电化学方法联用的一种新技术.本文介绍了SPR和EC-SPR的基本原理,并重点阐述了时间分辨SPR光谱技术与电化学方法联用及应用.该技术已广泛地应用于反应动态过程研究、生物化学传感器、电极/溶液界面的表征、动力学常数的测定以及生物分子相互作用等领域.
Surface Plasmon Resonance (SPR) is a very sensitive optical analysis method that uses the physical optical phenomena caused by the optical coupling of metal thin films. In recent years, Electrochemical Surface Plasmon Resonance (EC) -SPR) is a new technique that combines time-resolved surface plasmon resonance spectroscopy with electrochemical methods.This paper introduces the basic principles of SPR and EC-SPR, and focuses on time-resolved SPR spectroscopy and electrochemical methods This technique has been widely used in the fields of reaction dynamic process research, biochemical sensors, characterization of electrode / solution interface, determination of kinetic constants and biomolecular interactions.