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[目的]耳聋左慈丸干预老年肾虚耳聋小鼠血清TNF-α、IL-1β、IL-6。[方法]选SD大鼠60只,按随机数字表法随机分为3组,空白组,模型组、中药组,20只/组。腹腔注射氢化可的松造模,空白组、模型组:造模后5d给予生理盐水灌胃0.5 m L/只·d-1;中药组:造模后5d,按照鼠-人的折算剂量分别以不同药物灌胃,耳聋左慈丸汤剂按照中药水煎液制备,浓缩至0.3g/m L,0.5m L/只,灌胃22d。观测ABR听力,血清TNF-α、IL-1β和IL-6水平。[结果]ABR听力,血清TNF-α、IL-1β和IL-6水平改善中药组优于空白组、模型组。[结论]耳聋左慈丸可改善老年肾虚耳聋小鼠一般状态、降低ABR阈值、抑制TNF-α、IL-6、IL-1β表达。
[Objective] To investigate the effects of Deafness Zuoci Pill on serum TNF-α, IL-1β, IL-6 in aged kidney deafness mice. [Methods] Sixty SD rats were randomly divided into three groups according to random number table method: blank group, model group, TCM group and 20 / group. The rats in model group were injected intraperitoneally with hydrocortisone 0.5 mg / d · d -1 for 5 days after modeling, and the blank group and model group were given 0.5% To different drugs gavage, deafness Zuochu pill decoction prepared according to traditional Chinese medicine decoction, concentrated to 0.3g / m L, 0.5m L / only, gavage 22d. ABR hearing, serum TNF-α, IL-1β and IL-6 levels were observed. [Result] The improvement of ABR hearing, serum TNF-α, IL-1β and IL-6 levels in Chinese medicine group was better than that in blank group and model group. [Conclusion] Deafness Zuoxi Pill can improve the general status of aged kidney deafness mice, decrease ABR threshold, and inhibit the expression of TNF-α, IL-6 and IL-1β.