目的研究保胎无忧片的质量标准,建立其中厚朴酚与和厚朴酚含量的高效液相色谱测定方法。方法采用Hypersil ODS2(4.6 mm×250 mm,5μm)为色谱柱,甲醇-水(72∶28)为流动相,检测波长为294 nm。结果厚朴酚的线性范围为0.051~1.015μg,平均加样回收率为99.1%,RSD为0.74%;和厚朴酚的线性范围为0.010~0.208μg,平均加样回收率为98.0%,RSD为0.72%。结论本法简便、准确、灵敏可靠,可用于保胎无忧片的质量控制。 Objective To study the quality standard of Baoxuewuyou tablet and establish a HPLC method for the determination of honokiol and honokiol. Methods Hypersil ODS2 (4.6 mm×250 mm, 5 μm) was used as the column. Methanol-water (72:28) was used as the mobile phase and the detection wavelength was 294 nm. Results The linear range of honokiol ranged from 0.051 to 1.015 μg. The average recovery was 99.1% and the RSD was 0.74%. The linear range of honokiol was 0.010 to 0.208 μg, and the average recovery was 98.0%. It is 0.72%. Conclusion This method is simple, accurate, sensitive and reliable, and can be used for the quality control of Baotuoyao tablets.