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目的 在体外建立神经组织生长趋化性模型,探讨神经生长因子(NGF)对神经组织的趋化作用。 方法 直径约1mm的玻璃毛细管吸取约10μl的染料溶液,观察染料在培养液中的扩散;同样毛细管吸取含NGF的溶液与鸡胚神经节共培养,观察神经组织的生长方向。 结果 直径1mm的毛细管可稳定吸取10μl的液体;吸取的染料在毛细管周围形成分布均匀的浓度梯度;培养的神经节组织向高NGF浓度梯度方向生长。 结论 毛细管模型是研究神经组织趋化性生长的良好的模型;NGF对神经组织生长具有趋化作用。
Objective To establish a chemotactic model of neural growth in vitro and to explore the chemotactic effect of nerve growth factor (NGF) on nerve tissue. Methods Approximately 10 μl of the dye solution was drawn by a glass capillary of about 1 mm in diameter. The diffusion of the dye in the culture fluid was observed. Similarly, the solution containing NGF was co-cultured with the chick embryo ganglion to observe the growth direction of the nerve tissue. Results A capillary of 1 mm in diameter could stably draw 10 μl of liquid; the aspirated dye formed a uniformly distributed concentration gradient around the capillary; the cultured ganglion tissue grew toward a gradient of high NGF concentration. Conclusion The capillary model is a good model to study the chemotactic growth of neural tissue. NGF has a chemotactic effect on the growth of nerve tissue.