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目的:探讨乳腺癌组织中Runx3基因启动子甲基化及蛋白的表达与乳腺癌生物学行为的关系,并分析Runx3基因启动子区甲基化与其蛋白表达的相关性。方法:采用甲基化特异性PCR(methylation-specific PCR,MSP)技术和免疫组织化学SP法检测48例患者乳腺癌组织、18例癌旁乳腺组织中Runx3基因启动子甲基化情况及蛋白的表达。结果:Runx3基因启动子在乳腺癌组织及癌旁乳腺组织中的甲基化阳性率分别为52.1%和3.7%(P<0.05);Runx3蛋白在乳腺癌及癌旁乳腺组织中的阳性率分别为33.3%和92.6%(P<0.05);Runx3基因启动子甲基化及蛋白的表达分别与乳腺癌的组织学分级、临床分期及淋巴结转移有关(P<0.05);Runx3基因启动子甲基化与蛋白表达呈明显的负相关(P<0.05)。结论:Runx3基因启动子的异常甲基化是引起蛋白表达缺失的主要原因,Runx3基因启动子的异常甲基化与乳腺癌的发生、发展有关。
OBJECTIVE: To investigate the relationship between promoter methylation of Runx3 gene and the biological behavior of breast cancer in breast cancer and to analyze the correlation between Runx3 promoter methylation and its protein expression. Methods: Methylation-specific PCR (MSP) and immunohistochemistry (SP) were used to detect the promoter methylation of Runx3 and protein in 48 cases of breast cancer tissues and 18 cases of paracancer tissues expression. Results: The positive rates of Runx3 promoter methylation in breast cancer tissues and paracancer tissues were 52.1% and 3.7%, respectively (P <0.05). The positive rates of Runx3 in breast cancer tissues and paracancer tissues were (P <0.05). The promoter methylation and protein expression of Runx3 gene were correlated with histological grade, clinical stage and lymph node metastasis of breast cancer (P <0.05) There was a significant negative correlation between the expression and protein expression (P <0.05). Conclusion: Aberrant methylation of Runx3 promoter is the main reason for the loss of protein expression. Aberrant methylation of Runx3 promoter is associated with the occurrence and development of breast cancer.