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目的:研究蟾酥注射液对人耐阿霉素(ADM)的急性髓性白血病细胞HL60/ADM多药耐药(MDR)的逆转作用。方法:采用MTT法分别考察蟾酥注射液对HL60/ADM细胞增殖能力和对ADM敏感性的变化,并采用高效液相色谱法考察HL60/ADM细胞对ADM的摄取能力。结果:蟾酥注射液能抑制细胞增殖;非细胞毒剂量蟾酥注射液预作用后,ADM对HL60/ADM细胞的半数抑制浓度(IC50)显著降低(P<0.01),HL60/ADM细胞对ADM摄取能力显著增加(P<0.01)。结论:蟾酥注射液可部分逆转HL60/ADM细胞对ADM的耐药性。
Objective: To investigate the reversal effect of Toad injections on multidrug resistance (MDR) in human ADM-resistant acute myeloid leukemia cells HL60 / ADM. Methods: The proliferation of HL60 / ADM cells and the sensitivity to ADM were examined by MTT assay. The uptake of ADM by HL60 / ADM cells was investigated by high performance liquid chromatography. Results: Toad injections could inhibit the proliferation of HL60 / ADM cells after pretreatment with non-cytotoxic Danshensu injection (P <0.01), while the half-maximal inhibitory concentration (IC50) Significantly increased (P <0.01). Conclusion: Chansu injection can partially reverse the drug resistance of HL60 / ADM cells to ADM.