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目的 探讨一氧化氮（NO）和血管内皮生长因子（VEGF）在脑胶质瘤血管形成中的作用。方法 采用免疫组化法检测40例不同级别胶质瘤标本中的诱导型NO合酶（iNOS）、VEGF和Ⅷ因子相关抗原（FⅧRAg）的表达,分析其相互关系。结果①对照组无iNOS表达,胶质瘤组iNOS阳性表达率为62.5％,二者有显著性差异（P<0.001）;②iNOS阴性组微血管密度为（22.40±12.62）个·视野-1,iNOS阳性组为（36.90±22.21）个·视野-1,两者间有显著性差异（P<0.05）;③胶质瘤VEGF阳性表达率为65.0％,明显高于对照组（P<0.01）;④VEGF阳性组微血管密度为（38.09±21.69）个·视野-1,阴性组为（19.19±9.05）个·视野-1,两者间差异显著（P<0.01）;⑤iNOS阳性组中VEGF阳性细胞数明显高于iNOS阴性组（P<0.01）。结论 NO和VEGF参与了胶质瘤的血管形成及肿瘤生长,其具有重要的临床和病理学价值。
Objective To investigate the role of nitric oxide (NO) and vascular endothelial growth factor (VEGF) in glioma angiogenesis. Methods The expressions of iNOS, VEGF and Ⅷ factor related antigen (FⅧRAg) in 40 glioma specimens of different grades were detected by immunohistochemistry. The correlation was analyzed. Results ①No iNOS expression was found in the control group. The positive expression rate of iNOS in glioma group was 62.5% (P <0.001). ② The microvessel density in iNOS negative group was (22.40 ± 12.62) (36.90 ± 22.21) · field-1 in the positive group (P <0.05). The positive expression rate of VEGF in glioma was 65.0%, which was significantly higher than that in the control group (P <0.01). ④ The microvessel density in VEGF-positive group was (38.09 ± 21.69) · field-1, while in negative group was (19.19 ± 9.05) · field-1, the difference was significant (P <0.01); ⑤ The number of VEGF-positive cells in iNOS positive group Significantly higher than iNOS negative group (P <0.01). Conclusion NO and VEGF are involved in angiogenesis and tumor growth of gliomas, which have important clinical and pathological value.