论文部分内容阅读
目的制备在成熟成骨细胞中敲除基因fgfr1的特定细胞阶段性基因敲除小鼠。方法从美国NIH引进SPF级fgfr1条件性基因敲除(fgfr1flox/flox)小鼠,进行饲养、繁殖和PCR鉴定;fgfr1flox/flox小鼠与在成熟成骨细胞表达Cre重组酶的OC-Cre小鼠交配,获得在成熟成骨细胞中敲除一个基因拷贝的杂合子小鼠;杂合子小鼠之间交配,或者杂合子小鼠与fgfr1flox/flox小鼠交配,可以获得在成熟成骨细胞中敲除fgfr1基因的小鼠fgfr1△/△/OC-CreTG/+。结果成功制备在成熟成骨细胞中敲除fgfr1的细胞特异性敲除小鼠。结论成功引进fgfr1flox/flox小鼠,应用合理的基因条件性敲除繁殖策略,获得在成熟成骨细胞中敲除fgfr1的基因敲除小鼠。
Objective To prepare specific cell-stage knock-out mice knocked out of the gene fgfr1 in mature osteoblasts. METHODS: Fgfr1flox / flox mice were injected with SPF-fgfr1 gene from NIH for breeding, breeding and PCR. Fgfr1flox / flox mice and OC-Cre mice expressing Cre recombinase in mature osteoblasts Mating, obtaining heterozygous mice knocking out one copy of the gene in mature osteoblasts; mating heterozygous mice or mating mice with fgfr1flox / flox mice to obtain knockdown in mature osteoblasts Mouse fgfr1Δ / Δ / OC-CreTG / + except fgfr1 gene. Results Cell-specific knockout mice were successfully prepared that knocked out fgfr1 in mature osteoblasts. Conclusion The fgfr1flox / flox mice were successfully introduced and knockout fgfr1 knockout mice were obtained by using reasonable gene knockout breeding strategy.