Dear Editor,rnThe geminiviruses are small single-stranded plant DNA viruses belonging to the family Geminiviridae,which cause serious diseases in many economically important crops world-wide.Geminiviruses typically infect the differentiated leaf cells or vascular tissues,and multiply progeny DNA in the nucleus of infected cells.Geminiviruses encoding replication initiator protein (Rep) is essential for viral genome replication,but Rep does not possess DNA polymerase activity and relies heavily on host DNA replication machine for replication (Hanley-Bowdoin et al.2000).Therefore,exploring the host factors participating in geminivirus replication is of great significance in understanding geminivirus replication mechanism and demystifying the processes of plant DNA replication.However,many drawbacks in using plant host as a model block the research on the effects of host factors on viral DNA replication,for example,plants with some functional gene mutants lead to embryo lethal,the complexities of plant protoplasts transfection and culture inhibit the research progress,and so on.The existence of the above disadvantages urges us to seek a novel approach to identify and characterize the geminivirus-interacting host factors.