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近年来,由于生物化学、分子生物学和免疫学,特别是以单克隆抗体和细胞融合技术为代表的细胞工程学的发展,使对细菌毒素研究进入到分子水平。现不但能对毒素进行精密的分离、提纯、定性和分子结构分析,而且可通过对化学合成毒素结构的改造,进行分子结构与毒素作用相互关系的分析。由于分子水平的毒素生物膜受体和细胞内信息传递系统也逐渐明确,以及遗传工程技术已用于细菌活菌苗的开发,细菌毒素研究成果的应用已扩大到身体机能和生命科学等更大的范畴。本文着重介绍与小儿传染病关系较密切的毒素以及其分子结构和作用机理的最新研究动向与成果。一、白喉毒素白喉毒素的研究历史最长。由于仅微量
In recent years, due to biochemistry, molecular biology and immunology, especially the development of cellular engineering represented by monoclonal antibody and cell fusion technology, the study of bacterial toxins has been brought to the molecular level. Now not only the toxins can be accurately separated, purified, qualitative and molecular structure analysis, but also through the transformation of chemical synthesis of toxins, molecular structure and toxins interactions between the analysis. As molecular levels of toxin biofilm receptors and intracellular signaling systems have become clearer and genetic engineering techniques have been used to develop bacterial live vaccine strains, the application of bacterial toxin research has expanded to include greater body functions and life sciences Category. This article focuses on the latest research trends and achievements on the toxins that are closely related to pediatric infectious diseases and their molecular structure and mechanism of action. First, diphtheria toxin Diphtheria toxins research has the longest history. Because only a small amount