成骨细胞在骨组织生成上起着重要作用。当前,为了弄清骨折治愈、骨移植和异所性化骨等临床问题,对成骨细胞从细胞学、生理学和生化学方面做了大量研究。本文报道了作者用Wistar 种大鼠19～21日胚的颅顶骨中央部分分离的成骨细胞进行长达八周的培养观察。分离成骨细胞使用胶原酶。得到的成骨细胞在改良的Eagle’s MEM 培养基加上含有10% Osteoblasts play an important role in bone formation. At present, in order to find out the clinical problems of bone healing, bone transplantation and heterogenous bone formation, a great deal of studies have been done on osteoblasts in terms of cytology, physiology and biochemistry. Here we report the observation of osteoblasts isolated from the central part of the parietal bone of 19-21 embryos of Wistar rats for up to eight weeks. Isolation of osteoblasts using collagenase. The resulting osteoblasts were cultured in modified Eagle’s MEM medium supplemented with 10%