肾细胞培养是研究肾小管生理,病生及代谢调节的重要手段。我们建立了兔肾小管上皮细胞原代培养方法制作的体外庆大霉素(GM)损伤模型,采用流式细胞仪对其进行分析。观察了细胞分裂增殖指数(PI)、S期细胞敷、G_2M期细胞数变化及中药绞股蓝皂甙、AWS对GM肾毒性韵防治作用。肾小管上皮细胞培养及分离方法:将兔处死后无菌取肾,分离肾皮质,剪碎后移人80目钢网筛,研磨并用生理盐水冲洗于100目钢网筛上收集肾小管节段,离心后胰蛋白酶消化10min,中止消化后加入含10％小牛血清的RPM11640增养液,置于5％CO_2孵箱中培养、细胞贴壁后1天其外周开始长出细胞,6天左右细胞铺满培养瓶。取原氏培养第三天的细胞,倒去原培养液。实验分4组:(1)正常组即原代培养的肾小管细胞。(2)致伤组即暴露在含1mmol/L GM中的培养细胞。(3)绞股蓝皂甙组(GPS Renal cell culture is an important means to study the regulation of renal tubule physiology, pathogenesis and metabolism. We established an in vitro model of gentamicin (GM) injury induced by primary culture of rabbit renal tubular epithelial cells and analyzed by flow cytometry. The cell proliferation and proliferation index (PI), cell deposition in S phase, G 2 M phase cell number and Gynostemma pentaphyllum polysaccharides were observed. Renal tubular epithelial cell culture and isolation methods: The rabbits were sacrificed after taking the aseptic kidney, kidney cortex was isolated, cut and then moved to 80 mesh steel mesh, ground and washed with saline on the 100 mesh steel mesh to collect renal tubular segments , After centrifugation trypsin digestion 10min, stop digestion, add 10% fetal bovine serum RPM11640 nutrient solution, placed in 5% CO_2 incubator in culture, 1 day after adherent cells began to grow cells in the periphery, about 6 days Cells covered with culture bottles. Take the original culture of the third day of the cells, down to the original culture solution. The experiment was divided into 4 groups: (1) normal group that primary cultured tubular cells. (2) The injured group was exposed to cultured cells containing 1 mmol / L GM. (3) Gypenosides group (GPS