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目的：研究局部应用重组人干扰素－ａ２ｂ（ｒｈＩＦＮ－ａ２ｂ）软膏后有无透皮及其在皮肤内的浓度。方法：选用乳猪皮肤，体外固体制剂溶出度仪－旋转膜装置药物供给系统和体内给药系统，抗人ＩＦＮ－ａ单克隆抗体ＥＬＩＳＡ检测。结果：离体试验各时间点接受液中均未测得ｒｈＩＦＮ－ａ，而离体和体内试验皮肤匀浆中均能检测到ｒｈＩＦＮ－ａ。结论：ｒｈＩＦＮ－ａ２ｂ软膏能透入皮肤内，其在皮肤内的浓度达到该药在体外抑制Ｉ、ＩＩ型单纯疤疹病毒ＩＣ５０值的１．３－４．３和３．３－９．９倍。
OBJECTIVE: To investigate the presence or absence of transdermal and intradermal concentrations of rhIFN-a2b topical ointment. Methods: Superoxide dismutase (HRP) - drug delivery system and in vivo drug delivery system of swine porcine skin were used to detect anti-human IFN-a monoclonal antibody. RESULTS: No rhIFN-a was detected in the fluid received at all time points in vitro, whereas rhIFN-a was detected in both in vitro and in vivo skin homogenates. CONCLUSIONS: rhIFN-a2b ointment penetrates into the skin and its concentration in the skin achieves a 1.3-4.3 and 3.3-9.9 IC50 value of the herpes simplex I and II in vitro Times