【摘 要】
：
Mermithid nematodes,such as Ovomermis sinensis,are used as biologi-cal control agents against many insect pests,including cotton bollworm (Helicoverpa armigera).However,given the host\'s robust immune system,the infection rate of O.sinensis is low,thus
【机 构】
：
Hubei Key Laboratory of Genetic Regulation and Integrative Biology,School of Life Sciences,Central C
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Mermithid nematodes,such as Ovomermis sinensis,are used as biologi-cal control agents against many insect pests,including cotton bollworm (Helicoverpa armigera).However,given the host\'s robust immune system,the infection rate of O.sinensis is low,thus restricting its widespread use.To understand the host defense mech-anisms against mermithid nematodes,we identified and characterized a protein involved in the recognition of O.sinensis,the potential O.sinensis-binding protein C-type lectin 1 (HaCTL1a and/or HaCTL1b),which was eluted from the surface of O.sinensis af-ter incubation with H.armigera plasma.HaCTLlb is homologous to the previously re-ported HaCTL1a protein.HaCTL1 was predominantly expressed in hemocytes and was induced by the steroid hormone 20-hydroxyecdysone through ecdysone receptor (HaEcR)or ultraspiracle (HaUSP),or both.Binding assays confirmed the interactions of the HaCTL1 proteins with O.sinensis but not with Romanomermis wuchangensis,a para-sitic nematode of mosquito.Moreover,the HaCTL1 proteins were secreted into the hemo-coel and promoted hemocyte-mediated encapsulation and phagocytosis.A knockdown of HaEcR and/or HaUSP resulted in compromised encapsulation and phagocytosis.Thus,HaCTL1 appears to modulate cellular immunity in the defense against parasitic nema-todes,and the 20-hydroxyecdysone-HaEcR-HaUSP complex is involved in regulating the process.
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