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目的探讨增殖细胞核抗原(PCNA)在人脑胶质瘤中的表达与肿瘤生长、侵润、转移、复发的关系及在胶质瘤发生发展中的作用,拟为手术治疗及疗效提供客观依据。方法采用免疫组织化学染色方法检测正常脑组织及胶质瘤细胞中增殖细胞核抗原PCNA阳性表达率。结果对照脑组织增殖细胞核抗原表达阴性;不同级别胶质瘤细胞增殖细胞核抗原阳性表达率依次为WHOⅣ级100%(6/6)、WHOⅢ级94.44%(17/18)、WHOⅡ级62.50%(10/16)和WHOⅠ级42.86%(3/7);增殖细胞核抗原表达率与胶质瘤组织病理学分级呈正相关(rs=0.589,P=0.000);WHOⅢ-Ⅵ级与WHO I-Ⅱ级之间差异具有统计学意义(H=13.239,P=0.000)。结论胶质瘤组织中增殖细胞核抗原表达水平和肿瘤组织病理学分级呈正相关。提示,增殖细胞核抗原与胶质瘤恶性进展密切相关。
Objective To investigate the relationship between the expression of proliferating cell nuclear antigen (PCNA) in human glioma and the growth, invasion, metastasis and recurrence of gliomas and the role of PCNA in the development and progression of glioma. It is intended to provide an objective basis for surgical treatment and therapeutic effect. Methods Immunohistochemical staining was used to detect PCNA positive expression rate in normal brain tissue and glioma cells. Results The positive rate of proliferating cell nuclear antigen in control group was negative. The positive rate of proliferating cell nuclear antigen (PCNA) in different grades of glioma was 100% (6/6) in WHO Ⅳ, 94.44% (WHO) and 62.50% (WHO Ⅱ) / 16) and WHOⅠ-42.86% (3/7) respectively. The positive rate of proliferating cell nuclear antigen expression was positively correlated with the histopathological grade of glioma (rs = 0.589, P = 0.000) The difference was statistically significant (H = 13.239, P = 0.000). Conclusion The expression of proliferating cell nuclear antigen in glioma tissue is positively correlated with the histopathological grade. Tip, PCNA is closely related to the malignant progression of glioma.