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目的:观察合欢皮总皂苷对H22荷瘤小鼠CD4+和CD8+T淋巴细胞亚群、细胞因子白细胞介素-2(IL-2)和肿瘤坏死因子-α(TNF-α)水平,凋亡诱导因子(Fas),凋亡诱导因子配体(Fas L)和增殖细胞核抗原(PCNA)蛋白表达的影响,探讨其对细胞免疫功能的干预机制。方法:昆明种小鼠60只随机分为正常组、模型组、环磷酰胺组、合欢皮总皂苷高、中、低剂量组,建立小鼠H22荷瘤模型。给药组分别按环磷酰胺20 mg·kg-1·d-1,合欢皮总皂苷4,2,1 mg·kg-1·d-1ip,连续治疗11 d后,检测抑瘤率,血清IL-2,TNF-α水平,免疫组化染色检测肿瘤细胞Fas,Fas L和PCNA蛋白的表达;苏木素-伊红(HE)染色观察肿瘤组织形态。结果:与模型组比较,合欢皮总皂苷高、中、低剂量组小鼠移植瘤的平均质量均显著下降(P<0.05,P<0.01),合欢皮总皂苷中剂量组能明显提高CD4+T细胞,CD8+T细胞数量(P<0.05,P<0.01),对CD4+/CD8+无影响,合欢皮总皂苷中、高剂量组IL-2水平明显上升(P<0.05),TNF-α水平无变化,合欢皮总皂苷各剂量组Fas L,PCNA蛋白表达显著降低(P<0.01),中、高剂量组Fas表达水平显著升高(P<0.01)。结论:合欢皮总皂苷能够调节细胞免疫功能,其机制可能与其上调CD4+,CD8+T细胞亚群,促进IL-2分泌,提高Fas蛋白表达并抑制Fas L,PCNA蛋白表达有关。
Objective: To observe the effects of total Hesperidosides on CD4 + and CD8 + T lymphocyte subsets, interleukin-2 (IL-2) and tumor necrosis factor-α (TNF-α) On the expression of Fas, Fas L and PCNA proteins, and to explore the mechanism of its intervention on cellular immune function. Methods: Sixty Kunming mice were randomly divided into three groups: normal group, model group, cyclophosphamide group and HGH high, medium and low dose groups. H22 tumor-bearing mice model was established. The treatment groups were treated with cyclophosphamide 20 mg · kg-1 · d-1, total gypenosides 4, 2, 1 mg · kg-1 · d-1 ip, respectively, for 11 days. The expression of Fas, Fas L and PCNA were detected by immunohistochemical staining. The morphology of tumor was observed by hematoxylin-eosin staining. Results: Compared with the model group, the average quality of transplanted tumor of HGH group was significantly decreased (P <0.05, P <0.01), and the total dose of HGH group could significantly increase the CD4 + (P <0.05, P <0.01), but had no effect on CD4 + / CD8 +. The level of IL-2 in the middle and high dosage of Acorus gramineus saponins increased significantly (P <0.05) The changes of Fas L and PCNA protein were significantly decreased (P <0.01), and the expression of Fas in medium and high dose groups was significantly increased (P <0.01). CONCLUSION: Aconitine total saponin can modulate cellular immune function, which may be related to its upregulation of CD4 +, CD8 + T cell subsets, IL-2 secretion, Fas protein expression, and the inhibition of Fas L and PCNA protein expression.