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An electrochemical DNA sensor based on ferrocene-labelled peptide nucleic acid(PNA-Fc)was prepared.The hybridization between PNA-Fc and DNA immobilized on a gold electrode was examined by cyclic voltammetry(CV)and differential pulse volmmmetry fDPV).PNA-Fc shows a good electrochemically activity and has a redox potential of 170 mV verus Ag/AgCl electrode after hybridization,representing the characteristic of ferrocene/ferrocenium(Fc/Fc+)transformation.The results illustrate that PNA-Fc can be used as an effective electrochemical DNA probe sensor.