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目的:探讨黄芪多糖(APS)对高脂饲料诱导的大鼠胰岛素抵抗(IR)的治疗作用及机制。方法:将48只成年雄性SD大鼠随机分为正常对照组(NC组,12只)和高脂模型组(HFM组,36只),分别给予普通饲料和高脂饲料喂养。模型建立成功后,再次随机分组:高脂对照组(HFC组)、APS组和吡格列酮组(Pio组),每组均12只,分别给予生理盐水,200 mg.kg-1.d-1APS,20 mg.kg-1.d-1 Pio干预8周。随机选取各分组中的一半大鼠进行高胰岛素-正葡萄糖钳夹试验,以葡萄糖输注率(GIR)判定机体的胰岛素敏感性。同时测定另一半大鼠的空腹血糖(FBG),空腹胰岛素(FINS),游离脂肪酸(FFA),脂联素(APN)和抵抗素水平。结果:与NC组相比,HFC组大鼠血浆的FINS,FFA和抵抗素水平升高,GIR和APN水平降低(P<0.05);与HFC组相比,APS组大鼠血浆的FINS,FFA和抵抗素水平降低,APN水平和GIR升高(P<0.05)。GIR和血浆FINS、抵抗素、FFA浓度呈负相关,和血浆APN水平呈正相关(P<0.05)。结论:APS可以改善大鼠的胰岛素抵抗,可能与其升高血浆APN水平和降低血浆抵抗素有关。
Objective: To investigate the therapeutic effect and mechanism of astragalus polysaccharides (APS) on insulin resistance (IR) induced by high fat diet in rats. Methods: Forty eight adult male Sprague-Dawley rats were randomly divided into normal control group (n = 12) and hyperlipidemia model group (n = 36), fed with normal diet and high-fat diet respectively. After successful establishment of the model, rats in the HFC group, APS group and pioglitazone group (Pio group) were randomly divided into two groups randomly: normal saline, 200 mg.kg-1.d-1APS, 20 mg.kg-1.d-1 Pio intervention for 8 weeks. Half of the rats in each group were randomly selected for hyperinsulinemic-glucose clamp test to determine the insulin sensitivity of the body with glucose infusion rate (GIR). Fasting blood glucose (FBG), fasting insulin (FINS), free fatty acid (FFA), adiponectin (APN) and resistin levels were measured in the other half of the rats simultaneously. Results: Compared with NC group, the levels of FINS, FFA and resistin in plasma of HFC rats increased and the levels of GIR and APN decreased (P <0.05). Compared with HFC rats, FINS, FFA And resistin levels decreased, APN levels and GIR increased (P <0.05). GIR and plasma FINS, resistin, FFA concentration was negatively correlated, and plasma APN levels were positively correlated (P <0.05). Conclusion: APS can improve insulin resistance in rats, which may be related to the increase of plasma APN level and the decrease of plasma resistin.