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促血管生成素2(angiopoietin-2,Ang-2)是特异性血管生成刺激因子,仅在恶性肿瘤细胞中高表达,与肿瘤血管形成数量和预后关系密切。血清Ang-2对恶性肿瘤的早期诊断及发病机理研究有较高的临床应用价值。在表达并纯化Ang-2、制备Ang-2单抗的基础上,研制了Ang-2定量检测试剂盒(增强化学发光法),对其技术指标进行分析,并将该试剂盒应用于妇科恶性肿瘤的临床研究。结果表明,原核表达并纯化的Ang-2纯度≥92%;试剂盒灵敏度达0.1ng/ml;线性范围达0.5～25ng/ml;与脂蛋白、球蛋白、α1酸性糖蛋白、血红蛋白、粘蛋白、透明质酸、癌胚抗原不发生交叉反应;批内变异系数(CV)≤4.1%;妇科恶性肿瘤临床标本检验,宫颈癌阳性率为73.2%,卵巢癌阳性率为83.3%,子宫内膜癌阳性率为76.7%,其它妇科恶性肿瘤阳性率为70.4%,表明该试剂盒为恶性肿瘤的临床诊断奠定基础。
Angiopoietin-2 (angiopoietin-2) is a specific angiogenic stimulating factor that is only highly expressed in malignant tumor cells, which is closely related to the number and prognosis of tumor angiogenesis. Serum Ang-2 on the early diagnosis of malignant tumors and pathogenesis of high clinical value. On the basis of expressing and purifying Ang-2 and preparing Ang-2 monoclonal antibody, an Ang-2 quantitative detection kit (enhanced chemiluminescence method) was developed and its technical indicators were analyzed. The kit was applied to gynecological malignant Cancer research. The results showed that the purity of prokaryotic expression and purification of Ang-2 was ≥92%, the sensitivity of the kit was 0.1ng / ml, the linear range was 0.5 ~ 25ng / ml, and the affinity of lipoprotein, globulin, α1 acid glycoprotein, hemoglobin, , Hyaluronic acid and carcinoembryonic antigen do not cross-react; intra-assay coefficient of variation (CV) ≤4.1%; gynecologic malignancies clinical specimens tested, the positive rate of cervical cancer was 73.2%, the positive rate of ovarian cancer was 83.3% The positive rate of cancer was 76.7%, the positive rate of other gynecological malignancies was 70.4%, which indicated that this kit laid the foundation for the clinical diagnosis of malignant tumors.