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本文用琼脂培养法研究了新鲜和冷冻保存的骨髓粒细胞增殖活性动力学和分化特点。实验用CBA/H系小鼠股骨骨髓和健康人骨髓。先将冷凝后半固体琼脂扎些小孔,再向每皿加1毫升冷储的骨髓细胞悬液(含1×10~6个细胞),37℃、10%CO_2培养28天。刺激素为溶血自家血。48小时起用倒置显微镜计数灶、团数(10～20个细胞为小灶、10个以上为大灶,4～10个为细胞团)。然后将培养物用卡诺氏液固定,石腊和甲基纤维素包埋。组织片行
In this paper, agar culture was used to study the kinetics and differentiation characteristics of fresh and cryopreserved myelocytic proliferative activity. Experimental CBA / H mouse bone marrow and healthy human bone marrow. First, after the condensation semi-solid agar some holes, and then add 1 ml per dish cold storage of bone marrow cell suspension (containing 1 × 10 6 cells), 37 ℃, 10% CO 2 cultured for 28 days. Stimulating hormone hemolytic own blood. 48 hours with inverted microscope counting stove, the number of groups (10 to 20 cells for small lesions, 10 or more for the large stove, 4 to 10 for the cell group). Cultures were then fixed with Carnot’s solution, paraffin and methylcellulose embedding. Organization film line