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①目的调查青岛市区学龄前儿童巨细胞病毒感染情况。②方法用抗人巨细胞病毒（ＨＣＭＶ）早期抗原（ＥＡ）的单克隆抗体（ＭｃＡｂ），通过间接免疫过氧化物酶法，检测尿标本细胞培养中的ＨＣＭＶ－ＥＡ．③结果１９５名入托儿童尿标本中，有８９名（４５．６％）ＨＣＭＶ－ＥＡ检测阳性，其中１岁以下婴幼儿排毒率最低（１７．４％），１～２岁儿童排毒率最高（６５．６％）。９１名未入托的学龄前儿童中，有２１名（２３．１％）检测到ＨＣＭＶ，与幼儿园儿童排毒率（４５．６％）相比显著有差异性（χ２＝１３．３４７，Ｐ＜０．０１）。④结论青岛市区儿童主要在１～２岁感染ＨＣＭＶ，幼儿园内ＨＣＭＶ的传播流行主要由儿童间密切接触感染所致，这些排毒儿童对血清ＨＣＭＶ抗体阴性的育龄期妇女有较大危害，可能成为先天性ＨＣＭＶ感染的传染源
① Objective To investigate the status of cytomegalovirus infection in preschool children in Qingdao. MethodsMHMV-EA in urine samples was detected by indirect immunoperoxidase method using monoclonal antibody (McAb) against human cytomegalovirus (HCMV) early antigen (EA). Results The positive rate of HCMV-EA in 89 out of 195 children (45.6%) was the lowest (17.4%), and the detoxification rate was the highest in children 1-2 years old 65.6%). Of 91 non-care preschool children, 21 (23.1%) detected HCMV, which was significantly different from that of kindergarten children (45.6%) (χ2 = 13.347, P <0 .01). Conclusion The prevalence of HCMV infection in children aged 1 ~ 2 in Qingdao city is mainly caused by the close contact infection among children. These detoxified children are more harmful to women of childbearing age who are negative for serum HCMV antibody and may become Congenital HCMV infection source of infection