论文部分内容阅读
[目的]探讨复方三根制剂逆转K562/ADR、K562/VCR细胞株耐药性的机制。[方法]细胞K562、K562/ADR、K562/VCR培养于RPMI鄄1640安全培养液中。MTT法测定两株细胞的抗药性。RT鄄PCR法测定给药处理后24h、48h、72h后两耐药细胞株MDR1表达量。[结果]复方三根制剂和维拉帕米对K562/ADR和K562/VCR不同时段表达MDR1的量有显著差异。[结论]推测复方三根制剂逆转多药耐药机制为在转录水平下调MDR1mRNA,从而降低多药耐药细胞P鄄gp的表达。
[Objective] To investigate the mechanism of three compounds in reversing the drug resistance of K562 / ADR and K562 / VCR cell lines. [Methods] The cells K562, K562 / ADR and K562 / VCR were cultured in RPMI-1640 safe medium. MTT assay of two cell resistance. RT-PCR method was used to determine the expression of MDR1 in two drug-resistant cell lines 24h, 48h, 72h after treatment. [Results] There was a significant difference in the amount of MDR1 expression between K562 / ADR and K562 / VCR cells treated with the three preparations and verapamil. [Conclusion] It is speculated that the reversal of multidrug resistance mechanism of the three prescriptions of compound prescriptions is that MDR1 mRNA is down-regulated at the transcriptional level, thereby reducing the expression of P-gp in multidrug-resistant cells.