胡芦科植物苦瓜(Momordicacnarantia)新鲜的绿色果实在室温用乙醇匀类提取,过滤得到提取液,在40℃减压浓缩,然后在水,二氯乙烷,石油醚,甲醇,四氯化碳间用文献方法(Org chem 39(17):2477)分别提取。四氯化碳提取部分经反复、顺序连续的闪式柱层析,用不断变化极性的已烷—乙酸乙酯进行真空洗脱,以硅胶60TLC作对照,直到在TLC上获得纯的部分。酰基葡萄糖基甾醇是用3:7的乙酸乙酯—乙烷洗脱的并在分析的TLC上显示了Rf为 Fresh green fruits of the Momordicac narantia plant (Cumorculaceae) were extracted at room temperature with ethanol and filtered to obtain an extract, which was concentrated under reduced pressure at 40°C, then in water, dichloroethane, petroleum ether, methanol, carbon tetrachloride The respective methods were extracted by literature method (Org chem 39(17):2477). The carbon tetrachloride extracted fractions were subjected to repeated and sequential flash column chromatography and eluted with varying polarity of hexane-ethyl acetate in vacuo, using silica gel 60TLC as a control until a pure fraction was obtained on TLC. Acylglucosyl sterols were eluted with 3:7 ethyl acetate-hexane and Rf was shown on the analytical TLC.