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目的探讨急性一氧化碳中毒对大鼠脑红蛋白的影响,为一氧化碳中毒的防治提供理论依据。方法健康雄性SD大鼠20只随机分为对照组和一氧化碳染毒组,每组10只。染毒组按60ml/kg体重腹腔注射一氧化碳,每周一次,注射4周。对照组注射等量的空气。染毒结束后进行神经行为学测试包括旷场试验、甩尾试验和后肢撑力试验,同时检测大鼠海马、皮质、小脑中脑红蛋白的变化。结果一氧化碳中毒后大鼠在中央格停留时间明显增加,修饰次数、直立次数和大小便次数也较对照组明显增加,穿格次数明显减少;甩尾时间比对照组明显延长、后肢展开距离增加明显,差异有统计学意义。此外CO染毒大鼠皮质和小脑中的NGB含量增加,而海马中的NGB呈下降的趋势。结论一氧化碳中毒致脑组织脑红蛋白含量变化,这可能参与了CO中毒致神经损伤的机制。
Objective To investigate the effect of acute carbon monoxide poisoning on neuroglobin in rats and to provide a theoretical basis for the prevention and treatment of carbon monoxide poisoning. Methods Twenty healthy male SD rats were randomly divided into control group and carbon monoxide poisoning group, with 10 rats in each group. The rats in the exposure group were injected intraperitoneally with carbon tetrachloride (60ml / kg body weight) once a week for 4 weeks. The control group was injected with the same amount of air. Neurobehavioral tests after the end of the exposure included open-field test, tail-flick test and hindlimb support test, and the changes of neuroglobin in hippocampus, cortex and cerebellum of rats. Results After carbon monoxide poisoning, the residence time of rats in the central grid increased significantly, while the numbers of modification, erection and defecation increased significantly compared with those in the control group, and the number of wears significantly decreased. The flick time was significantly longer than that of the control group and the hindlimb expansion distance increased significantly ,The difference was statistically significant. In addition, the content of NGB in the cortex and cerebellum of CO-exposed rats increased, while the NGB in hippocampus showed a decreasing trend. Conclusions Carbon monoxide poisoning can change the content of brain tissue neuroglobin, which may be involved in the mechanism of nerve injury induced by carbon monoxide poisoning.