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[目的]观察养阴清热中药对C57BL/6小鼠移植Lewis瘤肺转移的作用,并探讨机理.[方法]干扰素和养阴清热中药分别作用于动物模型,21 d后处死动物,统计肺转移计数,免疫组化法检测瘤体血管内皮生长因子(VEGF)和微血管内皮标记物Factor-VⅢ.[结果]肺转移灶计数,中药组与模型组比较有显著差异(P<0.05),与干扰素组无显著差异(P>0.05),中药组在VEGF表达上与模型组比较有显著差异(P<0.05),与干扰素组无显著差异(P>0.05);中药组在肿瘤微血管密度(MVD)记数与模型组比较有显著差异(P<0.05),与干扰素组无显著差异(P>0.05).[结论]养阴清热法对L.L.C的转移有明显的抑制作用,其主要机理是在降低VEGF表达的基础上,抑制肿瘤血管生成.
[Objective] To observe the effect of Yangyin heat-clearing traditional Chinese medicine on lung metastasis of Lewis lung tumor in C57BL / 6 mice and to explore its mechanism. [Methods] Interferon and Yangyin Qingre TCM were respectively used in animal models. After 21 days, (P> 0.05). [Results] There were significant differences in the number of lung metastases between Chinese herbal medicine group and model group (P <0.05), and There was no significant difference between interferon group and interferon group (P> 0.05). There was significant difference (P <0.05) between the expression of VEGF and the model group in the traditional Chinese medicine group, and no significant difference with the interferon group (P> 0.05) (P <0.05), and there was no significant difference between MVD and interferon group (P> 0.05). [Conclusion] Yang-Yin heat-clearing method can obviously inhibit the metastasis of LLC, Mechanism is to reduce the expression of VEGF, based on the inhibition of tumor angiogenesis.