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目的 探讨大鼠急性吗啡镇痛耐受时 ,痛行为和海马ACTH和 β 内啡肽 (β EP)含量变化及重楼皂甙对其影响。方法 本实验采用大鼠急性吗啡镇痛耐受模型 ,运用热测痛法观察重楼皂甙对其痛阈的作用 ,并用放射免疫法测定海马ACTH和 β EP含量变化。 结果 在急性单次皮下注射盐酸吗啡 (6mg/kg)后 ,海马ACTH和 β EP含量升高 ,而当连续 5次皮下注射盐酸吗啡 (6mg/kg ,间隔 2h)后 ,海马ACTH和 β EP含量回降。重楼皂甙对这一回降过程有抑制作用 ,并增强吗啡对急性吗啡耐受大鼠痛阈的提高作用。结论 重楼皂甙在吗啡急性耐受形成后可能有增强吗啡镇痛的作用 ,并可能通过影响脑内ACTH和 β EP等因素影响耐受形成
Objective To investigate the changes of pain behavior, ACTH and β-EP in hippocampus and the effects of resplenin on acute morphine analgesia tolerance in rats. Methods The rat model of acute morphine analgesia tolerance was used in this study. The effect of polysaccharide on the pain threshold was observed by heat assay and the changes of ACTH and β EP in hippocampus were determined by radioimmunoassay. Results After acute single subcutaneous injection of morphine hydrochloride (6 mg / kg), the ACTH and β EP levels in the hippocampus increased. After 5 consecutive subcutaneous injections of morphine hydrochloride (6 mg / kg, 2 h intervals), ACTH and β EP contents Back down. Polygonum saponins can inhibit the fallback process and enhance the effect of morphine on the pain threshold of acute morphine-tolerant rats. Conclusions Saponins may enhance the analgesic effect of morphine after acute morphine formation and may affect the formation of tolerance by influencing factors such as ACTH and β EP in the brain