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目的建立鸡骨草肝炎颗粒的定性、定量方法。方法采用薄层色谱法定性鉴别处方中的鸡骨草,采用HPLC法测定制剂中的绿原酸和槲皮素。结果鸡骨草薄层鉴别方法分离度好、专属性强、简单可行;绿原酸和槲皮素的线性范围分别为0.0046~0.0276μg.μl-1(r=0.9999)、0.0015~0.0084μg.μl-1(r=0.9991),平均加样回收率分别为98.02%、97.91%,RSD=1.8%、1.1%。结论所建方法可控制鸡骨草肝炎颗粒的质量。
Objective To establish a qualitative and quantitative method of Abrupt Hepatitis Granule. Methods Thin layer chromatography was used to identify the prescription of Abrus cantoniensis. The contents of chlorogenic acid and quercetin in the preparations were determined by HPLC. Results The method for identifying thin layer of Abrus cantoniensis with good specificity and strong specificity was simple and feasible. The linear ranges of chlorogenic acid and quercetin were 0.0046-0.0276 μg.μl -1 (r = 0.9999) and 0.0015-0.0084 μg, respectively. μl-1 (r = 0.9991). The average recoveries were 98.02%, 97.91%, RSD = 1.8% and 1.1%, respectively. Conclusion The established method can control the quality of Abrupt Hepatitis particles.