Recent studies have found cold biases in a fraction of Argo profiles (hereinafter referred to as bad Array for Real-time Geostrophic Oceanography (Argo) profiles) due to the pressure drifts during 2003 and 2006. These bad Argo profiles have had an important impact on in situ observation-based global ocean heat content esti- mates. This study investigated the impact of bad Argo profiles on ocean data assimilation results that were based on observations from diverse ocean observation systems, such as in situ profiles (e.g., Argo, expendable bathy- thermograph (XBT), and Tropical Atmosphere Ocean (TAO), remote-sensing sea surface temperature products and satellite altimetry between 2004 and 2006. Results from this work show that the upper ocean heat content analysis is vulnerable to bad Argo profiles and demon- strate a cooling trend in the studied period despite the multiple independent data types that were assimilated. When the bad Argo profiles were excluded from the as- similation, the decreased heat content disappeared and a warming occurred. Combination of satellite altimetry and mass variation data from gravity satellite demonstrated an increase, which agrees well with the increased heat con- tent. Additionally, when an additional Argo profile quality control procedure was utilized that simply removed the profiles that presented static unstable water columns, the results were very similar to those obtained when the bad Argo profiles were excluded from the assimilation. This indicates that an ocean data assimilation that uses multiple data sources with improved quality control could be less vulnerable to a major observation system failure, such as a bad Argo event. Recent studies have found cold biases in a fraction of Argo profiles (hereinafter referred to as bad Array for Real-time Geostrophic Oceanography (Argo) profiles) due to the pressure drifts during 2003 and 2006. These bad Argo profiles have had an important impact on in situ observation-based global ocean heat content esti- mates. This study investigated the impact of bad Argo profiles on ocean data assimilation results that were based on observations from diverse ocean observation systems, such as in situ profiles (eg, Argo, expendable bathy - thermograph (XBT), and Tropical Atmosphere Ocean (TAO), remote-sensing sea surface temperature products and satellite altimetry between 2004 and 2006. Results from this work show that the upper ocean heat content analysis is vulnerable to bad Argo profiles and demon- strate a cooling trend in the study period despite the multiple independent data types that were assimilated. th the bad argo profiles were excluded from the as-similation, th combined with satellite altimetry and mass variation data from gravity satellite demonstrated an increase, which agrees well with the increased heat con- tent. removed the profiles that presented static unstable water columns, the results were very similar to those obtained when the bad Argo profiles were excluded from the assimilation. This indicates that an ocean data assimilation that uses multiple data sources with improved quality control could be less vulnerable to a major observation system failure, such as a bad Argo event.