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目的研究苦参碱对豚鼠心肌细胞动作电位时程和钾电流的影响,探讨其抗心律失常作用的可能机制。方法应用全细胞膜片钳技术记录心室肌细胞的动作电位时程和钾电流。结果在频率0.1Hz时,苦参碱100μmol.L-1以非频率依赖方式延长动作电位复极90%的时程达40%,在-120mV抑制内向整流钾电流(IK1)接近47%,减少快激活延迟整流钾电流的尾电流达50%,对慢激活延迟整流钾电流的尾电流无影响。结论苦参碱抗心律失常的机制可能与其抑制多种钾电流和延长动作电位时程有关。
Objective To study the effect of matrine on action potential duration and potassium current in guinea pig myocardium and explore the possible mechanism of its antiarrhythmic effect. Methods Whole cell patch clamp technique was used to record the action potential duration and potassium current of ventricular myocytes. RESULTS: At a frequency of 0.1 Hz, matrine at 100 μmol.L-1 prolonged the time course of the repolarization 90% by 40% in a non-frequency-dependent manner, and inhibited the inward rectifier potassium current (IK1) at -120 mV by nearly 47%. The tail current of the delayed rectifier potassium current is 50% faster, which has no effect on the tail current of the slow-activation delayed rectifier potassium current. Conclusion The anti-arrhythmic mechanism of matrine may be related to its inhibition of multiple potassium currents and prolonged action potential duration.