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目的分析斯氏狸殖吸虫童虫、成虫排泄分泌产物(ES)的蛋白条带和抗原性,并对两者进行免疫组织定位。方法十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)分离斯氏狸殖吸虫童虫ES蛋白、童虫虫体可溶性蛋白、成虫ES蛋白、成虫虫体可溶性蛋白,采用Western-blot方法分别用肺吸虫病人血清、小鼠抗血清识别特异性反应条带,并用酶标免疫组织化学技术对ES抗原进行组织定位。结果经SDS-PAGE分离的斯氏狸殖吸虫的虫体可溶性蛋白和ES蛋白,Western-blot结果显示,感染血清识别的童虫ES蛋白、成虫ES蛋白条带分别在28000、70000Mr和90000Mr处,小鼠抗血清识别的童虫ES蛋白、成虫ES蛋白条带分别在28000、70000Mr和45000、70000Mr处。童虫ES主要定位在后尾蚴的排泄囊,成虫ES主要定位在成虫肠腔上皮。结论 28000、70000Mr的童虫ES蛋白可能为斯氏狸殖吸虫的特异诊断抗原。
OBJECTIVE: To analyze the protein bands and antigenicity of the excretion and secretion products (ES) of Schistosoma penguingensis and the immunohistochemical localization of the two proteins. Methods SDS-PAGE was used to isolate ES protein, soluble protein of adult worms, ES protein of adult worms and soluble proteins of adult worms, blot method were used paragonimus patients serum, mouse antiserum specific reaction bands were identified, and enzyme-labeled immunohistochemistry of ES antigen tissue localization. Results The results of Western-blot showed that the ES protein and ES protein bands of Spermophilus serrata were 28,000, 70,000 and 90,000 Mr respectively, Mice ES protein, adult ES protein bands recognized by mouse antisera were at 28000, 700000 and 45000 and 700000 Mr, respectively. Schistosomula ES mainly located in the posterior cercaria discharge capsule, adult ES mainly located in adult intestinal lumen epithelium. Conclusion ES proteins of 28 000 and 700000 Mr may be specific diagnostic antigens of Trichoplusia.