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目的探讨增殖细胞核抗原(PCNA)、survivin基因与急性白血病(AL)的关系。方法收集2003—2004年浙江大学医学院附属第一医院住院AL患者的资料,采用免疫组化方法检测PCNA和RT-PCR方法检测survivin在AL在治疗前后以及与正常对照组的比较。结果初发AL患者与正常对照组的PCNA和survivin的表达差异有统计学意义(P<0.05)。AL在治疗前与治疗缓解后其PCNA的表达差异有统计学意义(P<0.05)。survivin高表达的急性髓细胞白血病(AML)缓解率低,但是急性淋巴细胞白血病(ALL)的缓解率与survivin表达无关。结论PCNA和survivin在白血病中均高表达,与白血病的发生及发展有一定的联系,PCNA与survivin互补,可作为判断急性白血病的疗效和预后指标。
Objective To investigate the relationship between proliferating cell nuclear antigen (PCNA) and survivin gene and acute leukemia (AL). Methods The data of AL patients hospitalized in the First Affiliated Hospital of Zhejiang University from 2003 to 2004 were collected. The expression of survivin in the AL was detected by immunohistochemistry and RT-PCR before and after treatment and compared with the normal control group. Results The expression of PCNA and survivin in primary AL patients and normal controls were significantly different (P <0.05). The expression of PCNA in AL before and after treatment was significantly different (P <0.05). Survivin high expression of acute myeloid leukemia (AML) remission rate is low, but the acute lymphoblastic leukemia (ALL) remission rate and survivin expression has nothing to do. Conclusions Both PCNA and survivin are highly expressed in leukemia, and have a certain relationship with the occurrence and development of leukemia. PCNA and survivin complement each other, which can be used as a prognostic indicator of acute leukemia.