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目的 :了解扬州地方株HPV16L1基因结构特点 ,为研制HPV16疫苗奠定基础。方法 :从感染HPV16的宫颈癌组织中提取DNA ,用PCR技术获得HPV16L1基因 ,以pGEM Teasy为克隆载体构建重组质粒pGEM T L1,进行限制性核酸内切酶及核苷酸序列分析。结果 :扬州地方株与德国标准株在核苷酸序列上有 7处不同 ,其中 4处由于核苷酸的改变 ,其编码氨基酸也相应发生变化 ,与标准株的同源性为 99.7% ;扬州地方株与中国株之间也存在 1至 2处核苷酸不同 ,但未造成氨基酸序列改变 ,其同源性为 99.9%。结论 :扬州地方株HPV16L1基因与标准株、中国株之间均存在差异 ,但与后者的差异极小 ,未造成氨基酸改变。
Objective: To understand the structural characteristics of HPV16 L1 gene in Yangzhou, and to lay the foundation for the development of HPV16 vaccine. Methods: DNA was extracted from cervical cancer tissues infected with HPV16. The HPV16 L1 gene was obtained by PCR. The recombinant plasmid pGEM T L1 was constructed by using pGEM Teasy as cloning vector. Restriction endonuclease and nucleotide sequence analysis were performed. Results: There were 7 different nucleotide sequences in the Yangzhou endemic strains and the German standard strains. Among them, four of them changed their encoded amino acids due to nucleotide changes, with 99.7% identity with the standard strains. Yangzhou There are also 1 to 2 nucleotide differences between the local strain and the Chinese strain, but no amino acid sequence change is caused. The homology is 99.9%. Conclusion: The difference between the HPV16L1 gene of Yangzhou endemic strain and that of the standard strain and the Chinese strain is very small, but the difference between the HPV16L1 gene and the Chinese strain is very small.