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目的调查小榄地区男性尿道人乳头状瘤病毒(HPV)感染及其基因亚型分析,为HPV感染分子流行病学提供依据。方法采集2012年8月-2013年9月小榄地区疑似HPV感染的350例男性患者尿道分泌物标本,采用凯普导流杂交HPV-DNA检测法进行21种HPV亚型检测。结果 350例男性患者送检标本中有131例检测HPV阳性,感染率为37.43%,共检测出19种HPV亚型,其中高危型占33.59%、中危型占9.16%、低危型占57.25%,HPV感染的高峰年龄为21~40岁,占90.84%,31~40岁年龄段患者HPV高危型与低危感染率差异无统计学意义,HPV感染基因亚型主要为11、6、16和CPA8304,检出频率分别42.75%、41.98%、13.74%和9.16%。结论小榄地区男性尿道HPV高危型感染较严重,感染好发于青中年男性,高危型感染常合并中危型或低危型感染,这对小榄地区HPV分子流行病学的研究具有重要意义。
Objective To investigate male urethrocytic human papillomavirus (HPV) infection and its subtypes in Siu Lam area and provide evidence for the molecular epidemiology of HPV infection. Methods Urine specimens from 350 male patients with suspected HPV infection in Xiaolan from August 2012 to September 2013 were collected and 21 HPV subtypes were detected by using the HPV-DNA detection method. Results Among the 350 male patients, 131 were positive for HPV testing, with an infection rate of 37.43%. A total of 19 HPV subtypes were detected, of which 33.59% were high-risk, 9.16% were medium-risk and 57.25% were low-risk %. The peak age of HPV infection was 21-40 years old, accounting for 90.84%. There was no significant difference in HPV high-risk and low-risk infection rates between 31-40 years old. The HPV subtypes were 11,6,16 And CPA8304, the detection frequency was 42.75%, 41.98%, 13.74% and 9.16% respectively. Conclusions High risk HPV infection in male urethra is more serious in Siu Lam area. Infection occurs in young middle-aged males. High-risk infection is often associated with moderate-risk or low-risk infection, which is important for the study of HPV molecular epidemiology in Siu Lam significance.