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目的:建立用小微粒色谱柱-高效液相色谱法来测定花生中黄曲霉毒素的方法。方法:样品打碎均匀后,用Bond Elut PH柱萃取,以V水∶V甲醇∶V乙腈=50∶40∶10为流动相,经小微粒色谱柱分离,紫外检测器检测。结果:四种黄曲霉毒素G2、G1、B2、B1得到很好的分离.检测限分别为:0.5μg/kg0、.8μg/kg、0.2μg/kg、0.4μg/kg。花生样品中黄曲霉毒素G2、G1、B2、B1在添加浓度为0.3μg/kg～3μg/kg的范围内,平均回收率在:82%～96%。相对标准偏差在1.2%～3.3%。结论:该法能在短时间内使花生中黄曲霉毒素G2、G1、B2、B1得到很好的分离,准确,比国标法快捷,值得推广。
Objective: To establish a method for the determination of aflatoxins in peanut using small particle column - high performance liquid chromatography. Methods: The sample was homogenized and extracted with Bond Elut PH column. The mobile phase was V water: V methanol: V acetonitrile = 50:40:10, separated by small particle column and detected by UV detector. Results: The four aflatoxins G2, G1, B2 and B1 were well separated.The detection limits were 0.5μg / kg0, 0.8μg / kg, 0.2μg / kg and 0.4μg / kg, respectively. The average recoveries of aflatoxins G2, G1, B2 and B1 in peanut samples were in the range of 0.3μg / kg ~ 3μg / kg at 82% ~ 96%. The relative standard deviation was 1.2% ~ 3.3%. Conclusion: This method can make aflatoxin G2, G1, B2, B1 in peanut be well separated in a short period of time, which is faster than national standard method and worth popularizing.