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对１０例龋坏引起的慢性牙髓炎、未穿髓的新鲜离体患牙，进行分段定位、半薄切片、革兰氏染色，光镜观察定位后，再行超薄切片，醋酸铀－柠檬酸铅复染，电镜进行超微结构观察。结果：（１）患牙牙髓组织中有５例观察到多种类型微生物存在，为球杆菌、球菌、杆菌。（２）牙髓组织中的细菌位于细胞内及细胞外，并处于各种状态，有生长繁殖阶段的也有固缩溶解阶段的。（３）深龋引起的长期有症状的慢性牙髓炎牙髓组织中有大量活细菌存在，细菌均存在于冠髓内，接近龋坏部位，周围及深层组织中未见菌体。并观察到组织细胞吞噬大量细菌后，细胞变性，出现核固缩、核仁消失，细胞膜不清楚。细胞周围有大量溶酶体出现。结果提示：深龋引起的长期有症状的慢性牙髓炎，尽管未穿髓，牙髓组织中，仍可有大量活细菌长期存在。
10 cases of chronic pulpitis caused by caries, non-pierced freshly isolated teeth were segmented, semi-thin sections, Gram stain, light microscopy and positioning, and then ultra-thin slices, uranyl acetate - lead citrate double staining, electron microscopy ultrastructure observation. Results: (1) Five kinds of dental pulp tissues were observed in many kinds of microorganisms, such as coccus, coccus and bacillus. (2) The bacteria in the dental pulp tissue are located in the cells and outside the cells, and are in various states. There are also stages of pyknosis and dissolution in the stages of growth and reproduction. (3) Long-term symptomatic chronic pulpitis caused by deep caries There are a large number of live bacteria in dental pulp. Bacteria are found in the coronal pulp, close to the carious sites, and there is no bacterial cells in the surrounding and deep tissues. And observed a large number of cells after phagocytosis of cells, the cell degeneration, nuclear pyknosis, nucleolus disappear, the cell membrane is not clear. A large number of lysosomes around the cells appear. The results suggest that long-term symptomatic chronic pulpitis caused by deep caries may still have a large number of viable bacteria in the dental pulp tissue, although not worn.