通过对实验室已获得的1 685个橡胶炭疽病菌T-DNA插入突变体的生物学及致病性测定,从中筛选出15个致病力明显减弱的突变体,并进行PCR检测。结果表明,该基因组中均含潮霉素磷酸转移酶基因片段。在PDA培养基上,其中2个突变体菌落颜色异常,6个生长速率下降,8个产孢量显著降低,2个突变体分生孢子形态异常,2个附着胞形态异常,3个不能形成附着胞,在洋葱表皮上,侵染钉形成率显著降低。 Fifteen mutants with significantly reduced pathogenicity were screened out from the biological and pathogenicity tests of 1 685 T-DNA insertion mutants in laboratory, and PCR was performed. The results showed that the genome contains hygromycin phosphotransferase gene fragment. On the PDA medium, two of the mutant colonies were abnormal in color, with 6 growth rates decreasing, 8 sporulation significantly reduced, 2 conidia morphologically abnormal, 2 adherent cells morphologically abnormal, 3 could not form Appressorium, on the onion epidermis, significantly reduced the rate of infection of the formation of nails.