本实验在完成ＩＬ－２基因转染瘤苗的基础上，探索了重组ＩＦＮ－γ对其效果的增强作用。结果表明，ＩＬ－２基因转染使Ｂ１６细胞成瘤性显著降低；小鼠经ＩＬ－２基因修饰的Ｂ１６细胞免疫后，对接种的亲代Ｂ１６细胞有保护作用；ＩＬ－２基因转染的Ｂ１６细胞对接种亲代Ｂ１６细胞的小鼠有一定的治疗作用，与重组ＩＦＮ－γ联合应用后，治疗作用加强。上述结果表明，佐以合适的重组细胞因子，有可能提高单基因转染瘤苗的作用，是一条值得探索的途径 In this study, based on the completion of IL-2 gene-transfected tumor vaccine, the effect of recombinant IFN-γ on enhancing its effect was explored. The results showed that the transfection of IL-2 gene significantly reduced the tumorigenicity of B16 cells. After immunization with IL-2 gene-modified B16 cells, the transplanted B16 cells with IL-2 gene had a protective effect. The cells have a therapeutic effect on the mice inoculated with parental B16 cells and the therapeutic effect is strengthened after the combination with recombinant IFN-γ. The above results show that, with appropriate recombinant cytokines, may enhance the role of single-gene transfection vaccine is a worth exploring ways