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用LR White树脂低温包埋感染人恶性疟原虫FCC1/HN株的红细胞,用保护性单克隆抗体F6-D3和F6-C2并结合蛋白A-胶体金探针免疫标记恶性疟原虫红内期185kDa和82/41kDa蛋白。结果表明单克隆抗体F6-D3识别的185kDa蛋白定位于游离的和细胞内的裂殖子表面以及未成熟裂殖体的细胞质、质膜及带虫泡膜。而单克隆抗体F6-C2识别的82/41 kDa蛋白则定位于未成熟裂殖体及成熟裂殖子的棒状体中。从超微结构上表明185 kDa和82/41 kDa保护性抗原分别为恶性疟原虫FCC1/HN株的裂殖子表面抗原和裂殖子棒状体抗原。
Erythrocytes infected with P. falciparum FCC1 / HN strain were stained with LR White resin at low temperature. Immuno-labeling of Plasmodium falciparum erythropoietic 185 kDa with protective monoclonal antibodies F6-D3 and F6-C2 and binding protein A-colloidal gold probe And 82/41 kDa protein. The results show that the 185 kDa protein recognized by the monoclonal antibody F6-D3 is localized on the free and intracellular merozoites as well as on the cytoplasm, plasma membrane and the vacuolar membrane of immature schizonts. While the 82/41 kDa protein recognized by the monoclonal antibody F6-C2 is localized in the rod-like bodies of immature and mature merozoites. From the ultrastructure it is shown that the 185 kDa and 82/41 kDa protective antigens are merozoite surface antigen and merozoite rod antigen of P. falciparum FCC1 / HN strain, respectively.