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用反向间接血凝法、噬菌体技术和喷菌法,对安徽全椒、芜湖、石台、合肥等地区上年水稻白叶枯病重病田内和田边的若干种越冬后的植物作带菌检测,结果表明:茭白(Zizanin caduciflora Hand-Mazz)、看麦娘(Alopecurus aequalis Sobol)、菵草(Beckmannia syzigachne(Seud.)Fern)、鹅观草(Roegneria kamojz Ohwi)、艾蒿(Artemisia rulgaris Linn)、紫云英(Astragalus sinicus Linn)等植物带有白叶枯病菌。带菌量的大小,因带菌植物的种类和样品采集的地点而异。在检测的带菌植物中,带菌量较高的有茭白、紫云英等。另用白叶枯菌对多种杂草进行剪叶接菌,经13～20天后,茭白、看麦娘、游草等禾本科杂草的叶片上表现明显症状。杂草病叶的浸泡液与兔抗白叶枯菌的致敏血球产生特异性凝集反应。用噬菌体法也检测到大量活菌;接种稻叶又引起水稻发病。说明在被接种杂草上感染的是白叶枯细菌。
By reverse indirect hemagglutination, bacteriophage technology and spraying method, we carried out the detection of several overwintering plants in the areas of Heilongjiang, Anhui, such as Quanjiao, Wuhu, Shitai, Hefei, The results showed that: Zizanin caduciflora Hand-Mazz, Alopecurus aequalis Sobol, Beckmannia syzigachne (Seud.) Fern, Roegneria kamojz Ohwi, Artemisia rulgaris Linn, Astragalus sinicus Linn and other plants with bacterial blight. The amount of fungi can vary depending on the type of fungus and the location of sample collection. In the test with the contaminated plants, with a higher amount of bacterin white, asparagus and so on. Another use of Bacillus to cut a variety of weeds on the leaf-cutting bacteria, after 13 to 20 days, Zhaobai, see Ophiopogon, grass and other grass weeds on the leaves showed obvious symptoms. The soaking solution of weeds diseased leaves and rabbit erythroblasts allergic blood cells produce specific agglutination reaction. A large number of viable bacteria were also detected by phage method. Inoculation of rice leaves also caused the rice disease. Bacteriocin is indicated in the infected weed.