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试验材料为邯郸羊角椒。将制备的花粉播在10%蔗糖浓度加50ppm硼酸的培养基上,培养7小时镜检萌发情况。温度是影响花粉萌发的重要条件,25—27℃是花粉萌发的最适温度。低于10℃和高于40℃则不萌发。不同花龄花粉的生活力相差很大。开花当日花粉生活力最强,开花前一日的次之,开花后一日的花粉生活力明显降低。在有硅胶的干燥内置4℃冰箱中贮藏5天以后的花粉仍有50%的萌发率,而在室内自然条件下保存2—3日则生活力明显下降。因此,辣椒杂交制柙工作应在6月中旬,每日上午6—9时进行为宜,此时的田间气温和空气相对湿度均有利于杂交授粉。杂交座果率高。采集开花当日花粉,蕾期去雄同时授粉。
Test materials for Handan sheep horn. The prepared pollen sown in 10% sucrose concentration plus 50ppm boric acid medium, cultured 7 hours microscopic germination. Temperature is an important condition for pollen germination, 25-27 ℃ is the optimum temperature for pollen germination. Below 10 ° C and above 40 ° C, no germination occurs. The vitality of different age-pollen vary greatly. The pollen viability was the strongest on the day of flowering, the day before flowering was second, the pollen viability on the first day after flowering was significantly reduced. Pollen germination was still 50% after 5 days of storage in a refrigerator with a silica gel at 4 ° C. However, the viability of the pollen decreased by 2-3 days after being stored in a natural room for 2 to 3 days. Therefore, the pepper hybrid system 柙 work should be carried out in mid-June, 6-9 am daily appropriate, at this time field temperature and air relative humidity are conducive to cross-pollination. The rate of crossbreeding is high. Collection of flowering day pollen, buds to the same time pollination.