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肿瘤转移抑制蛋白(Metastasis suppressor1,Mtss1),又名肿瘤转移消失蛋白(Missing in metastasis,MIM)在小脑神经元发育中受到调控,依次表达两种亚型:包含Src磷酸化位点的广泛表达亚型;含Src磷酸化位点片段经RNA剪接去除的神经元特异亚型.为检测这两种Mtss1亚型的酪氨酸磷酸化水平是否因Src磷酸化位点的去除存在明显区别,制备了灵敏度较高并可特异性沉淀外源和内源表达的Mtss1的兔多克隆抗体,对发育时期与成年大鼠小脑内源Mtss1酪氨酸磷酸化水平进行检测,发现成年后的Mtss1与出生后发育时期的Mtss1均发生明显的酪氨酸磷酸化,表明剪接去除包含有Src磷酸化位点中的神经元特异亚型中,还有其他的酪氨酸残基被磷酸化,提示其他酪氨酸磷酸化激酶信号通路对Mtss1神经元亚型的调控作用.
Metastasis suppressor1 (Mtss1), also known as Missing in metastasis (MIM), is regulated in the development of cerebellar neurons and has two subtypes: the extensively expressed subunit containing the Src phosphorylation site Type; a neuron-specific subtype removed by RNA splicing containing Src phosphorylation site fragments To test whether the tyrosine phosphorylation levels of these two Mtssl subtypes are significantly different from the removal of the Src phosphorylation site, Sensitive and can specifically precipitate exogenous and endogenous expression of Mtss1 rabbit polyclonal antibody to development and adult rat cerebellum endogenous Mtss1 tyrosine phosphorylation level was detected in adult Mtss1 and after birth Significant tyrosine phosphorylation of Mtss1 occurred during the developmental stages, indicating that there are other tyrosine residues phosphorylated in the splicing and removal of neuron-specific subtypes including Src phosphorylation sites, suggesting that other tyrosine Regulation of Acid Phosphokinase Signal Pathway on Mtss1 Neuron Subtypes.