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目的:观察新型抗癫癇药氨己烯酸的视网膜毒性与光毒性的关系,探索氨己烯酸视网膜损伤的预防机制。方法:选用30只健康雄性BALB/c小鼠。正常治疗组(n=10),置于正常饲养环境中(黑暗与光照交替12h),给予氨己烯酸250mg··-1腹腔注射;暗室治疗组(n=10),24h置于暗室环境中,药物治疗浓度同正常治疗组;对照组(n=10),置kg-1d于正常饲养环境中,给予0.9%NaCl0.1mL·-1,腹腔注射。1个月后处死小鼠取视网膜进行5种抗体(Anti-VGLUT、Anti-dBsaaoon、Anti-Goα、Anti-PKCα和Anti-calbindin)的免疫荧光染色。结果:5种抗体染色表明暗室治疗组小鼠的视杆细胞突触、ON-双极细胞突触、水平细胞突触由视网膜外丛状层(OPL)向外核层(ONL)的退缩在暗室环境中可被抑制。结论:氨己烯酸的小鼠视网膜毒性有光依赖性,去除光毒性的影响,小鼠视网膜第一与第二级神经元之间的病理性突触改变可被完全抑制。
OBJECTIVE: To observe the relationship between retinal toxicity and phototoxicity of a new antiepileptic drug, and to explore the mechanism of prevention of retinoic acid damage. Methods: Thirty healthy male BALB / c mice were selected. The normal treatment group (n = 10) was placed in the normal feeding environment (alternating darkness and light for 12 hours), given intraperitoneal injection of amino acid 250mg ·· -1; darkroom treatment group (n = 10) (N = 10). The rats in the control group (n = 10) were given 0.9% NaCl (0.1 mL · L -1) and injected intraperitoneally with kg-1d in the normal feeding environment. One month later, the mice were sacrificed and the retina was taken for immunofluorescence staining of five kinds of antibodies (Anti-VGLUT, Anti-Goalpha, Anti-Goalpha, Anti-PKCa and Anti-calbindin) RESULTS: Five antibody staining showed that the retina synapses, ON-bipolar synapses, horizontal synapses in darkroom-treated mice retarded from the extrapulmonary plexus (OPL) to the outer nuclear layer (ONL) Dark room environment can be suppressed. CONCLUSION: The retinal toxicity of aminohexanoic acid is light-dependent, and the phototoxicity is eliminated. The pathological synaptic changes between the first and the second neurons in the mouse retina can be completely inhibited.