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目的: 探讨乳腺癌组织中细胞核体积与相关临床病理特征之间的关系; 方法: 收集55例乳腺癌标本的归档蜡块, 作连续切片, 8μm切片1张和相邻4μm切片4张, 行Feulgen染色;用细胞图像分析仪测定每例标本8μm切片的细胞核体积, 并比较与相关临床病理特征、雌孕激素受体及其DNA含量的关系; 结果: 8μm切片所测得的体积比4μm薄切片所测得的体积要大; 不同病理分型、不同组织学分级、不同临床分期、不同淋巴结转移状态、以及不同ER表达状态的样本细胞核体积有显著性差异; 165个样本的细胞核体积与DNA指数呈正的直线相关关系。结论: 8μm切片可以获得较为完整的细胞核体积; 细胞核体积可以作为判断肿瘤恶性度的较为客观的指标。
Objective: To investigate the relationship between cell nuclear volume and related clinicopathological features in breast cancer tissues. METHODS: A total of 55 paraffin blocks from breast cancer specimens were collected for continuous sectioning, 1 slice at 8 μm and 4 slices at 4 μm adjacent to Feulgen. Staining; The volume of nuclei in each sample 8 μm section was determined with a cell image analyzer, and the relationship with the relevant clinicopathological features, estrogen and progesterone receptors and their DNA content was compared; Results: The volumetric ratio of 4 μm thin sections was measured at 8 μm sections. The measured volume was large; there was a significant difference in the size of the sample nuclei between different pathological types, different histological grades, different clinical stages, different lymph node metastases, and different ER expression states; nuclear volume and DNA index of 165 samples. Positive linear relationship. Conclusion: The 8μm slice can obtain a relatively complete nuclei volume. The nuclei volume can be used as an objective index to judge the malignancy of the tumor.