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目的:观察鼻炎清颗粒治疗大鼠变应性鼻炎(AR)后鼻粘膜组织形态学变化和细胞因子水平,探讨该药治疗AR的部分免疫学机制。方法:用卵清白蛋白、氢氧化铝、百白破疫苗联合致敏大鼠制成变应性鼻炎动物模型,将鼻炎清颗粒灌胃给药2周后,观察鼻粘膜组织学变化,用ELISA法检测血清中细胞因子IL-4、IFN-r水平。结果:鼻炎清颗粒治疗组中大剂量组和中剂量组血清1L-4水平明显低于模型组,差异有统计学意义(P<0.01~0.05);IFN-r水平明显高于模型组,差异有统计学意义(P<0.01);光镜观察发现,模型组鼻粘膜上皮细胞脱落、水肿、血管扩张、腺体增生,固有层内可见大量嗜酸性粒细胞、肥大细胞浸润,用药组中大剂量组和中剂量组及阴性对照组则无上述改变。结论:鼻炎清颗粒通过调节Th1和Th2细胞因子的表达,纠正失衡的Th1/Th2的细胞因子网络并抑制炎症细胞在鼻粘膜的汇集,防止其脱颗粒,对变应性鼻炎产生治疗作用。
OBJECTIVE: To observe the nasal mucosal morphological changes and cytokines levels after treatment of rhinitis granules in rats with allergic rhinitis (AR), and to explore the immunologic mechanisms of this drug in the treatment of AR. METHODS: Animal models of allergic rhinitis were prepared by using sensitized rats combined with ovalbumin, aluminum hydroxide, and Peridotama vaccine. After 2 weeks of intragastric administration of Biyanqing granules, the histological changes of nasal mucosa were observed and ELISA was used. Methods Serum levels of cytokines IL-4 and IFN-r were measured. Results: The serum 1L-4 levels in the high-dose group and the middle-dose group of the Biyanqing granules treatment group were significantly lower than the model group, the difference was statistically significant (P<0.01~0.05); the IFN-r level was significantly higher than the model group, the difference There was statistical significance (P<0.01). Light microscopy showed that the model group had epithelial cell shedding, edema, vasodilation, and glandular hyperplasia in the model group. A large number of eosinophils and mast cells infiltrated in the lamina propria. The above changes were not observed in the dose group and the middle dose group and the negative control group. Conclusion: Biyanqing granules can correct the expression of Th1 and Th2 cytokines, correct imbalanced Th1/Th2 cytokine network and inhibit the collection of inflammatory cells in the nasal mucosa, prevent their degranulation, and produce therapeutic effects on allergic rhinitis.