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目的了解吉氏染色法对疟原虫染色效果的影响因素,寻找出最佳的染色条件。方法比较吉氏染色法不同染色时间、不同染液浓度以及染液中颗粒性杂质的去除对外周血中疟原虫的染色情况,寻找最佳效果。结果在本实验室自制吉氏染液薄血膜染色的最佳染色时间和最佳染色浓度分别为10min、1∶5稀释;15min、1∶10稀释;20min、1∶15稀释以及25min、1∶20稀释;厚血膜染色5min就能达到最佳结果。自制吉氏原液经离心后再稀释染色,染色效果比未离心的原液好,镜下观察杂质少而干净。结论吉氏染色法对疟原虫的染色效果的影响因素有染色时间和染液浓度等,相关实验室应予关注。
Objective To understand the factors that affect the staining effect of Plasmodium by Chrysalis staining and find out the best staining conditions. Methods The best results were obtained by comparing the staining of malaria parasite in peripheral blood with different staining time, different dye concentration and the removal of granular impurities in the dye solution. Results The optimal staining time and the optimal staining concentration for the thin-film staining of homemade jixi dye in our laboratory were 10min and 1: 5 respectively; 15min and 1:10 dilution; 20min and 1:15 dilution and 25min, 1 : 20 dilution; thick film staining 5min will be able to achieve the best results. Self-made Ji’s original solution after centrifugation and then diluted staining, staining effect than non-centrifuged solution is good, microscopic observation of impurities less and clean. Conclusions The factors affecting the staining effect of P. pastoris on Chrysoplasm are staining time and concentration of dye solution. The relevant laboratories should pay attention.