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目的:探讨重组蛋白转导域-寡聚化域-血凝素(protein transduction domain-oligomerization domain-hemagglutinin,PTD-OD-HA)对慢性粒细胞白血病(chronic myeloid leukemia,CML)BaF3-P210细胞小鼠致瘤能力的影响。方法:将BaF3-P210细胞和经40μmol/L PTD-OD-HA处理48h的BaF3-P210细胞分别经尾静脉注射入BALB/c小鼠体内,观察小鼠一般状况以及生存时间,计数外周血白细胞,外周血和骨髓涂片进行Wright-Giemsa染色,肝、脾和肺等各主要脏器组织进行HE染色,蛋白质印迹法检测小鼠骨髓细胞bcr/abl蛋白的表达。结果:BaF3-P210细胞组和经PTD-OD-HA处理的BaF3-P210细胞组小鼠CML发病率分别为90%(9/10)和80%(8/10),外周血白细胞计数分别为(44.3±4.8)×109/L和(20.6±3.2)×109/L(P<0.05),骨髓细胞中bcr/abl癌蛋白表达量分别为5.13±0.46和1.32±0.29(P<0.05),平均生存期分别为(101.3±6.2)d和(185.4±8.7)d(P<0.05)。Wright-Giemsa染色可见,经PTD-OD-HA处理的BaF3-P210细胞组小鼠骨髓、肝脏和脾脏中的白血病细胞浸润程度比BaF3-P210细胞组下降。结论:经PTD-OD-HA处理后的BaF3-P210细胞在BALB/c小鼠体内的致白血病潜能明显受到抑制。
Objective: To investigate the effect of PTD-OD-HA on BaF3-P210 cells in chronic myeloid leukemia (CML) Effect of tumorigenic ability of mice. Methods: BaF3-P210 cells and BaF3-P210 cells treated with 40μmol / L PTD-OD-HA for 48h were injected into BALB / c mice via caudal vein respectively. The general condition and survival time of mice were observed. Peripheral blood leucocytes , Peripheral blood and bone marrow smears were stained with Wright-Giemsa, liver, spleen and lungs and other major organs of HE staining, Western blotting detection of mouse bone marrow cells bcr / abl protein expression. Results: The incidence of CML in BaF3-P210 cells and BaF3-P210 cells treated with PTD-OD-HA was 90% (9/10) and 80% (8/10), respectively. The peripheral blood leukocyte counts were (44.3 ± 4.8) × 109 / L and (20.6 ± 3.2) × 109 / L respectively (P <0.05). The expression of bcr / abl oncoprotein in bone marrow cells were 5.13 ± 0.46 and 1.32 ± 0.29 (P <0.05) Mean survival was (101.3 ± 6.2) d and (185.4 ± 8.7) days, respectively (P <0.05). The Wright-Giemsa staining showed that leukemia cells in the bone marrow, liver and spleen of the BaF3-P210-treated mice treated with PTD-OD-HA decreased compared with those of the BaF3-P210 cells. CONCLUSION: The leukemia potential of BaF3-P210 cells treated with PTD-OD-HA in BALB / c mice was significantly inhibited.