本文以澳大利亚桉树林和松树林的土壤为例 ,采用Napp提取液和SDS直接溶解土壤细菌 ,并配合温浴 -玻璃珠震荡、苯酚 -氯仿萃取和异丙醇提取以及纯化DNA等步骤 ,直接从土壤样品中提取了土壤细菌DNA。所得DNA完全适用于酶解和PCR扩增的要求。该方法高效简单 ,费用低 ,在土壤微生物研究中具有重要的应用价值 Taking the soil of Australian eucalyptus forest and pine forest as an example, the Napp extract and SDS were used to dissolve the soil bacteria directly. With the steps of warm bath-glass bead shaking, phenol-chloroform extraction and isopropanol extraction and DNA purification, Soil bacterial DNA was extracted from the samples. The resulting DNA is fully suitable for enzymatic and PCR amplification requirements. The method is simple and efficient, has low cost and has important application value in the research of soil microorganism